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The molecular and functional characterization of single cells at scale has emerged as a key driver to unravel tissue biology. Thus, it is important to understand the strengths and limitations of transcriptomic approaches, molecular barcoding and functional assays used to study cellular properties at the single-cell level. Here, we review recent relevant work from the haematopoietic system and discuss how to interpret and integrate data obtained with different technologies.

Original publication

DOI

10.1038/s41556-018-0227-8

Type

Journal article

Journal

Nat Cell Biol

Publication Date

01/2019

Volume

21

Pages

2 - 8

Keywords

Animals, Cell Differentiation, Cell Lineage, Gene Expression Profiling, Hematopoiesis, Hematopoietic Stem Cells, Humans, Reproducibility of Results, Single-Cell Analysis