Equipment
Cell Analysers
ATTUNE NXT
The facility has 3 Attune NxT cell analysers. All are equipped with 4 lasers and can assay up to 14 colours plus FSC and SSC. They are all equipped with auto samplers for direct sampling from 96-well and 384-well plates. Attune 3 is equipped with the Cytkick Max autosampler – this can run all standard microplate formats, deep well plates and tube racks. The unique feature of the Attune NxT is acoustic focussing, which allows samples to be run faster without losing resolution.
The Attune NxT is available in Standard and alternative Violet filter configurations.
Attune Startup and Shutdown Procedure (with Autosampler)
Attune Startup and Shutdown Procedure (without Autosampler)
LSR FORTESSA
The BD LSR Fortessa is a 4 laser instrument that is capable of measuring 15 colours plus FSC and SSC. It can be equipped with a high throughput sampler (HTS) which allows sampling directly from microplates.
Click here for more details of the LSR Fortessa optical configuration and parameters
Fortessa Startup and Shutdown Procedure
LSR FORTESSA X20
The Facility has two Fortessa X20s. X20-1 is a 4 laser instrument capable of measuring 16 colours plus FSC and SSC. X20-2 has an additional UV laser and can measure 18 colours plus FSC and SSC. They can be equipped with a high throughput samplers (HTS) which allow sampling directly from microplates.
Click here for more details of the LSR Fortessa X20-1 optical configuration and parameters
Click here for more details of the LSR Fortessa X20-2 optical configuration and parameters
Fortessa X20 Startup and Shutdown Procedure
LSR FORTESSA X50 (SYMPHONY A5)
The X50 is a 5 laser analyser that is capable of measuring 30 colours plus FSC and SSC. It can be equipped with a high throughput sampler (HTS) which allows sampling directly from microplates. The X50 has the potential to be upgraded with more lasers to give a maximum of 48 colours. The optical configuration is complementary to the Facility’s FACSymphony S6 cell sorter
Click here for more details of the LSR Fortessa X50 optical configuration and parameters
Cell Sorters
BD FACSDISCOVER S8 CELL SORTER
BD FACSDiscover™ S8 Cell Sorter with BD CellView™ Image Technology and BD SpectralFX™ Technology, the first spectral flow cytometer sorter with sort-capable image analysis, expands the power of cell analysis and sorting to new dimensions by combining spectral flow cytometry with real-time spatial and morphological insights.
BD FACSARIA III
The BD FACSAria III is a 4 laser 15 colour sorter housed inside a Class I BSC for enhanced operator protection. It can sort into a variety of tubes and plates, including single cell indexed sorts.
Click here for the Aria III optical configuration and parameters
FACSARIA FUSION
The BD FACSAria Fusion is a 4 laser 16 colour sorter housed inside a Class II BSC for enhanced operator and sample protection. It can sort into a variety of tubes and plates, including single cell indexed sorts.
Click here for the Fusion optical configuration and parameters
SONY MA900
The Sony MA900 is a fully automated cell sorter with 4 lasers and is capable of sorting up to 12 colours plus FSC and BSC (SSC). The Blue (488nm) and Yellow Green (561nm) lasers are focused onto the same excitation point (colinear). The Violet (405nm) and Red (640nm) are focused onto a second excitation region so it is important to be aware of the spectral properties of the fluorochromes that are used. Up to four way sorting is possible into various tubes and one–way sorting is possible into plates of up to 384 wells.
Click here for the MA900 optical configuration and parameters
Sony MA900 Startup and Shutdown Procedure