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MicroRNAs are important posttranscriptional regulators of gene expression, which have been shown to fine-tune innate immune responses downstream of pattern recognition receptor (PRR) signaling. This study identifies miR-650 as a novel PRR-responsive microRNA that is downregulated upon stimulation of primary human monocyte-derived dendritic cells (MDDCs) with a variety of different microbe-associated molecular patterns. A comprehensive target search combining in silico analysis, transcriptional profiling, and reporter assays reveals that miR-650 regulates several well-known interferon-stimulated genes, including IFIT2 and MXA. In particular, downregulation of miR-650 in influenza A infected MDDCs enhances the expression of MxA and may therefore contribute to the establishment of an antiviral state. Together these findings reveal a novel link between miR-650 and the innate immune response in human MDDCs.

Original publication

DOI

10.1002/eji.201444970

Type

Journal article

Journal

Eur J Immunol

Publication Date

01/2016

Volume

46

Pages

167 - 177

Keywords

Dendritic cells, Host/pathogens interactions, ISGs, Innate immunity, MicroRNA, Cells, Cultured, Dendritic Cells, Down-Regulation, Flow Cytometry, Gene Expression Regulation, Humans, Immunity, Innate, Immunoblotting, Influenza A virus, MicroRNAs, Microscopy, Confocal, Myxovirus Resistance Proteins, Oligonucleotide Array Sequence Analysis, Receptors, Pattern Recognition, Signal Transduction, Transfection