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Nonsyndromic cleft lip with or without palate (CL/P) is thought to be caused by the interplay of genetic and environmental factors, and this has thus hindered the process of identifying genetic causative factors. Numerous studies in the past decade have implicated IRF6 in CL/P, but this has not often been replicated in other populations. In specific, the only etiologic single-nucleotide polymorphism (SNP) identified in the IRF6 locus (rs642961) has recently been shown not to be associated with CL/P in diverse populations. We therefore used a genewide tagging SNP (tagSNP) haplotyping approach (including rs642961 as a tagSNP) to detect all potential risk-conferring haplotypes and combined this with detailed subphenotyping of CL/P cases (N = 150) according to severity. We observed a significant overrepresentation of a tagSNP haplotype carrying the rs642961 risk allele in the most severe subphenotype of CL/P (complete bilateral CL/P; P = 0.008, odds ratio = 4.97, 95% confidence interval = 1.33 to 18.46). It was recently shown that >80% of IRF6 mutations in syndromic CL/P occur on the same haplotype background. We therefore suggest that IRF6 is a marker of CL/P severity.

Original publication




Journal article


J Dent Res

Publication Date





226S - 232S


congenital, genetic heterogeneity, haplotypes, medical genetics, odds ratio, single nucleotide polymorphism, Adenine, Biomarkers, Tumor, Case-Control Studies, Chromosome Mapping, Cleft Lip, Cleft Palate, Gene Frequency, Genetic Variation, Genome-Wide Association Study, Genotyping Techniques, Guanine, Haplotypes, Humans, Interferon Regulatory Factors, Linkage Disequilibrium, Mutation, Phenotype, Polymorphism, Single Nucleotide, Retrospective Studies, Thymine