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We have combined the circular chromosome conformation capture protocol with high-throughput, genome-wide sequence analysis to characterize the cis-acting regulatory network at a single locus. In contrast to methods which identify large interacting regions (10-1000 kb), the 4C approach provides a comprehensive, high-resolution analysis of a specific locus with the aim of defining, in detail, the cis-regulatory elements controlling a single gene or gene cluster. Using the human α-globin locus as a model, we detected all known local and long-range interactions with this gene cluster. In addition, we identified two interactions with genes located 300 kb (NME4) and 625 kb (FAM173a) from the α-globin cluster.

Original publication




Journal article


Philos Trans R Soc Lond B Biol Sci

Publication Date





chromatin conformation capture, cis-regulatory elements, α-globin locus, CCCTC-Binding Factor, Chromatin Assembly and Disassembly, Chromosomes, Human, Pair 16, Gene Regulatory Networks, Genetic Loci, Genome, Human, Humans, Open Reading Frames, Promoter Regions, Genetic, Protein Interaction Mapping, Regulatory Sequences, Nucleic Acid, Repressor Proteins, alpha-Globins, beta-Globins