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Global gene expression profiling of highly purified 5q-deleted CD34+CD38(-)Thy1+ cells in 5q- myelodysplastic syndromes (MDSs) supported that they might originate from and outcompete normal CD34+CD38(-)Thy1+ hematopoietic stem cells. Few but distinct differences in gene expression distinguished MDS and normal stem cells. Expression of BMI1, encoding a critical regulator of self-renewal, was up-regulated in 5q- stem cells. Whereas multiple previous MDS genetic screens failed to identify altered expression of the gene encoding the myeloid transcription factor CEBPA, stage-specific and extensive down-regulation of CEBPA was specifically observed in MDS progenitors. These studies establish the importance of molecular characterization of distinct stages of cancer stem and progenitor cells to enhance the resolution of stage-specific dysregulated gene expression.

Original publication




Journal article



Publication Date





3005 - 3014


ADP-ribosyl Cyclase 1, Aged, Aged, 80 and over, Antigens, CD34, CCAAT-Enhancer-Binding Proteins, Cell Lineage, Chromosomes, Human, Pair 5, Female, Gene Expression, Gene Expression Profiling, Hematopoietic Stem Cells, Humans, Image Processing, Computer-Assisted, In Situ Hybridization, Fluorescence, Male, Middle Aged, Myelodysplastic Syndromes, Nuclear Proteins, Polycomb Repressive Complex 1, Polymerase Chain Reaction, Proto-Oncogene Proteins, RNA, Repressor Proteins, Thy-1 Antigens