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Expression microarrays have great potential for clinical use but variability of the results represents a challenge for reliable practical application. The amount of fluorescent dye used in microarray experiments is a significant source of variability that has not been systematically studied. Here we demonstrate that the quantity of Cy3 dye affects microarray results performed on tumor specimens. Signal-to-noise ratios and coefficients of variation are significantly improved by increasing Cy3 to 150-180 pmol, but any further increase does not improve the data. In conclusion, optimal amounts of dye reduce variability and improve reliability of expression microarray experiments.

Original publication




Journal article


Mol Biotechnol

Publication Date





151 - 154


Carbocyanines, Fluorescent Dyes, Gene Expression Profiling, Humans, Neoplasms, Oligonucleotide Array Sequence Analysis