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The African swine fever virus (ASFV) j4R protein is expressed late during the virus replication cycle and is present in both the nucleus and the cytoplasm of infected cells. By using the yeast two-hybrid system, direct binding, and coprecipitation from cells, we showed that the j4R protein binds to the alpha chain of nascent polypeptide-associated complex (alpha NAC). Confocal microscopy indicated that a proportion of j4R and alpha NAC interact in areas close to the plasma membrane, as well as through the cytoplasm in cells. In vitro binding studies suggested that binding of j4R to alpha NAC did not interfere with the binding of alpha- and beta NAC subunits (the BTF3 transcription factor).

Original publication

DOI

10.1128/jvi.76.19.9991-9999.2002

Type

Journal article

Journal

J Virol

Publication Date

10/2002

Volume

76

Pages

9991 - 9999

Keywords

African Swine Fever Virus, Animals, Cell Nucleus, Chlorocebus aethiops, Cytoplasm, Molecular Chaperones, Nuclear Proteins, Open Reading Frames, Trans-Activators, Transcription Factors, Transfection, Two-Hybrid System Techniques, Vero Cells, Viral Proteins