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The Saccharomyces cerevisiae Rmi1 protein is a component of the highly conserved Sgs1-Top3-Rmi1 complex. Deletion of SGS1, TOP3, or RMI1 is synthetically lethal when combined with the loss of the Mus81-Mms4 or Slx1-Slx4 endonucleases, which have been implicated in Holliday junction (HJ) resolution. To investigate the causes of this synthetic lethality, we isolated a temperature-sensitive mutant of the RMI1 strain, referred to as the rmi1-1 mutant. At the restrictive temperature, this mutant phenocopies an rmi1Δ strain but behaves like the wild type at the permissive temperature. Following a transient exposure to methyl methanesulfonate, rmi1-1 mutants accumulate unprocessed homologous recombination repair (HRR) intermediates. These intermediates are slowly resolved at the restrictive temperature, revealing a redundant resolution activity when Rmi1 is impaired. This resolution depends on Mus81-Mms4 but not on either Slx1-Slx4 or another HJ resolvase, Yen1. Similar results were also observed when Top3 function was impaired. We propose that the Sgs1-Top3-Rmi1 complex constitutes the main pathway for the processing of HJ-containing HRR intermediates but that Mus81-Mms4 can also resolve these intermediates.

Original publication

DOI

10.1128/MCB.01130-10

Type

Journal article

Journal

Mol cell biol

Publication Date

05/2011

Volume

31

Pages

1921 - 1933

Keywords

DNA, Bacterial, DNA, Cruciform, DNA-Binding Proteins, Endonucleases, Escherichia coli Proteins, Flap Endonucleases, Holliday Junction Resolvases, Methyl Methanesulfonate, Mutation, Recombination, Genetic, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Temperature