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Tri-C is a chromosome conformation capture (3C) approach that can efficiently identify multiway chromatin interactions with viewpoints of interest. As opposed to pair-wise interactions identified in methods such as Hi-C, 4C, and Capture-C, the detection of multiway interactions allows researchers to investigate how multiple cis-regulatory elements interact together in higher-order structures in single nuclei and address questions regarding structural cooperation between these elements. Here, we describe the procedure for designing and performing a Tri-C experiment.

Original publication




Journal article


Methods Mol Biol

Publication Date





95 - 112


Chromosome conformation capture, Gene regulation, Genome organization, Multiway interactions, cis-regulatory elements, Chromatin, Chromosomes, Regulatory Sequences, Nucleic Acid