Chromosome Conformation Capture with Nuclear Titrated Capture-C (NuTi Capture-C)
Downes DJ., Hughes JR.
<jats:title>Abstract</jats:title> <jats:p>NuTi Capture-C is a Chromosome Conformation Capture (3C) approach, which can very efficiently identify chromatin interactions at target viewpoints at high resolution. The addition of high-throughput sequencing adaptors prior to enrichment allows for multiplexing of replicates and comparison samples. This method is an improvement on the previous NG Capture-C<jats:sup>1</jats:sup> method in that modifications have been made to the <jats:italic>in situ</jats:italic> 3C method to improve nuclear integrity (Nuclear 3C). Additionally, capture has been optimised to viewpoint complexity through titration, maximising on target sequence specificity. The experiment will take several weeks and provide reproducible interaction profiles for tens to thousands of viewpoints of interest.</jats:p>