Alastair Smith
Postdoctoral Researcher
I joined the Milne lab in 2018 after completing my PhD in the lab of Joan Boyes at the University of Leeds in 2018, where I investigated the temporal regulation of V(D)J recombination. My current research investigates the emergence of novel enhancers in leukaemia, with a focus on MLL-AF4 Acute Lymphoblastic Leukaemia (ALL).
I use a wide variety of high-throughput techniques (ChIP-seq, low cell number ChIPMentation, ATAC-seq, RNA-seq and Capture-C ) together with machine learning and other bioinformatic methods to better understand the regulation of chromatin and gene expression.
Recent publications
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A human genome editing-based MLL::AF4 B-cell ALL model recapitulates key cellular and molecular leukemogenic features.
Journal article
Bueno C. et al, (2023), Blood
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Temporal analyses reveal a pivotal role for sense and antisense enhancer RNAs in coordinate immunoglobulin lambda locus activation.
Journal article
Gao Z. et al, (2023), Nucleic Acids Res
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Dysregulation of chromatin via H3K27 methylation underpins differentiation arrest in Isocitrate dehydrogenase-mutant Acute Myeloid Leukaemia
Preprint
Silveira DRA. et al, (2023)
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MLL-AF4 cooperates with PAF1 and FACT to drive high-density enhancer interactions in leukemia.
Journal article
Crump NT. et al, (2023), Nat Commun, 14
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Author Correction: Capture-C: a modular and flexible approach for high-resolution chromosome conformation capture.
Journal article
Downes DJ. et al, (2023), Nat Protoc