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A system devised for tissue typing the human leukocyte antigen-A (HLA-A) locus from genomic DNA by the polymerase chain reaction (PCR) has been used to investigate abnormalities of HLA expression in a panel of 30 cell lines derived from colorectal adenocarcinomas, by comparison of the HLA-A locus genotype with surface expression of HLA. Eleven cell lines gave single HLA-A locus specificity on PCR typing, suggesting that loss of HLA alleles is a common abnormality. In one of these cell lines the loss of an HLA-A locus allele was confirmed by comparison with DNA from a lymphoblastoid B cell line derived from the same patient. In three cell lines, loss of expression of an HLA-A locus determinant was observed in spite of the presence of the relevant allele in genomic DNA. Three cell lines showed absent HLA expression associated with failure to express beta 2-microglobulin. These data indicate that at least three independent mechanisms were involved in the loss of HLA expression on the colorectal tumor cell lines.

Type

Journal article

Journal

J Immunother Emphasis Tumor Immunol

Publication Date

10/1993

Volume

14

Pages

163 - 168

Keywords

Antigens, Surface, Chromosome Mapping, Colorectal Neoplasms, Cytotoxicity, Immunologic, Genome, Genotype, HLA Antigens, HLA-A Antigens, HLA-A2 Antigen, Histocompatibility Antigens Class I, Humans, Immunotherapy, Polymerase Chain Reaction, Tumor Cells, Cultured