Low-input Capture-C: A Chromosome Conformation Capture Assay to Analyze Chromatin Architecture in Small Numbers of Cells.

Oudelaar AM., Downes DJ., Davies JOJ., Hughes JR.

Chromosome conformation capture (3C) techniques are crucial to understanding tissue-specific regulation of gene expression, but current methods generally require large numbers of cells. This protocol describes two new low-input Capture-C approaches that can generate high-quality 3C interaction profiles from 10,000-20,000 cells, depending on the resolution used for analysis.

DOI

10.21769/BioProtoc.2645

Type

Journal article

Journal

Bio Protoc

Publication Date

05/12/2017

Volume

7

Keywords

Capture-C, Chromatin architecture, Chromosome conformation capture (3C), Low input, Nuclear organization, Small cell numbers

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