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Endocytosis is essential for uptake of many substances into the cell, but how it links to nutritional signalling is poorly understood. Here, we show a new role for endocytosis in regulating the response to low phosphate in Schizosaccharomyces pombe. Loss of function of myosin I (Myo1), Sla2/End4 or Arp2, proteins involved in the early steps of endocytosis, led to increased proliferation in low-phosphate medium compared to controls. We show that once cells are deprived of phosphate they undergo a quiescence response that is dependent on the endocytic function of Myo1. Transcriptomic analysis revealed a wide perturbation of gene expression with induction of stress-regulated genes upon phosphate starvation in wild-type but not Δmyo1 cells. Thus, endocytosis plays a pivotal role in mediating the cellular response to nutrients, bridging the external environment and internal molecular functions of the cell.

Original publication

DOI

10.1242/jcs.171314

Type

Journal article

Journal

J cell sci

Publication Date

15/10/2015

Volume

128

Pages

3707 - 3713

Keywords

Endocytosis, Myosin, Phosphate sensing, Actin-Related Protein 2, Endocytosis, Gene Deletion, Gene Expression Regulation, Fungal, Myosin Heavy Chains, Phosphates, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Signal Transduction, Transcriptome