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The aorta-gonad-mesonephros (AGM) region is a potent hematopoietic site in the midgestation mouse conceptus and first contains colony-forming units-spleen day 11 (CFU-S(11)) at embryonic day 10 (E10). Because CFU-S(11) activity is present in the AGM region before the onset of hematopoietic stem cell (HSC) activity, CFU-S(11) activity in the complex developing vascular and urogenital regions of the AGM was localized. From E10 onward, CFU-S(11) activity is associated with the aortic vasculature, and is found also in the urogenital ridges (UGRs). Together with data obtained from organ explant cultures, in which up to a 16-fold increase in CFU-S(11) activity was observed, it was determined that CFU-S(11) can be increased autonomously both in vascular sites and in UGRs. Furthermore, CFU-S(11) activity is present in vitelline and umbilical vessels. This, together with the presence of CFU-S(11) in the UGRs 2 days before HSC activity, suggests both temporally and spatially distinct emergent sources of CFU-S(11). (Blood. 2000;96:2902-2904)

Type

Journal article

Journal

Blood

Publication Date

15/10/2000

Volume

96

Pages

2902 - 2904

Keywords

Animals, Animals, Outbred Strains, Aorta, Cell Lineage, Crosses, Genetic, Female, Gestational Age, Gonads, Hematopoiesis, Extramedullary, Hematopoietic Stem Cells, Male, Mesonephros, Mice, Mice, Inbred C57BL, Mice, Inbred CBA, Microscopy, Electron, Scanning, Radiation Chimera