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Endoplasmic reticulum (ER)-resident proteins are continually retrieved from the Golgi and returned to the ER by Lys-Asp-Glu-Leu (KDEL) receptors, which bind to an eponymous tetrapeptide motif at their substrate's C terminus. Mice and humans possess three paralogous KDEL receptors, but little is known about their functional redundancy, or if their mutation can be physiologically tolerated. Here, we present a recessive mouse missense allele of the prototypical mammalian KDEL receptor, KDEL ER protein retention receptor 1 (KDELR1). Kdelr1 homozygous mutants were mildly lymphopenic, as were mice with a CRISPR/Cas9-engineered frameshift allele. Lymphopenia was cell intrinsic and, in the case of T cells, was associated with reduced expression of the T-cell receptor (TCR) and increased expression of CD44, and could be partially corrected by an MHC class I-restricted TCR transgene. Antiviral immunity was also compromised, with Kdelr1 mutant mice unable to clear an otherwise self-limiting viral infection. These data reveal a nonredundant cellular function for KDELR1, upon which lymphocytes distinctly depend.

Original publication

DOI

10.1073/pnas.1515619112

Type

Journal article

Journal

Proc natl acad sci u s a

Publication Date

20/10/2015

Volume

112

Pages

E5706 - E5714

Keywords

N-ethyl-N-nitrosourea, T-cell development, T-cell survival, lymphocytes, positive selection, Animals, Chronic Disease, Endoplasmic Reticulum, Female, Genetic Predisposition to Disease, Lymphopenia, Male, Mice, Mice, Mutant Strains, Mutation, Receptors, Peptide, Virus Diseases