Addendum: Precise tuning of gene expression levels in mammalian cells.

Michaels YS., Barnkob MB., Barbosa H., Baeumler TA., Thompson MK., Andre V., Colin-York H., Fritzsche M., Gileadi U., Sheppard HM., Knapp DJHF., Milne TA., Cerundolo V., Fulga TA.

Following re-sequencing of the miSFIT constructs used in the paper, two of the construct variants inserted into the 3'UTR of PD-1, namely '12C' and '17A, 18G', have been found to contain additional insertions not present in the other construct variants. The data points corresponding to these constructs in Figs. 2c, f and Supplementary Fig. 9 are therefore no longer valid. However the overall conclusion that step-wise control over gene expression levels using the miSFIT constructs remains unaffected by these errors. Updated versions of Fig. 2 and Supplementary Fig. 9 are presented in the accompanying Addendum.

DOI

10.1038/s41467-019-10615-0

Type

Journal article

Journal

Nat Commun

Publication Date

10/06/2019

Volume

10

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