Our range of microscopes offers imaging solutions fitting most research needs. From widefield microscopy to the most advanced STED super-resolution and photon-counting technology, our users have access to hardware working in optimal condition and the expertise needed to profit from it. We also strive to implement new imaging protocols and techniques in a collaborative manner.
Leica TCS SP8 STED 3X inverted microscope
This system, mounted on an inverted microscope stand, is a flexible, multipurpose platform.
In its simplest form it is a laser-scanning confocal that has two standard PMTs and three more sensitive HyD detectors. One of these HyDs is additionally an SMD detector for single molecule detection and photon counting applications (FLIM, FCS). In order to fully profit from the SMD, the system is complemented with a PicoQuant photon counting module and the corresponding software analysis tools.
This system offers full excitation flexibility, boasting a pulsed “White Light Laser” (WLL) that can be tuned at any wavelength in the 470-670 nm range. The system further has a 405 nm diode laser, an Argon laser (ideal for FRAP), and a pulsed 440 nm laser. These, in combination with the Leica AOBS-based detection, mean that any fluorophore can be efficiently excited and that any emission spectrum can be captured.
The main feature of the system is the STED (Stimulated Emission Depletion) super-resolution capability. This imaging technique achieves 50 nm lateral resolution for 2D STED (xy-donut) or 130 nm lateral and axial resolution for 3D STED (xy and z-donuts). We have superb 100x oil and 93x glycerol high NA lenses specifically designed for STED on the system. While the pulsed WLL enables excitation, the powerful, doughnut-shaped 775, 660 and 592 nm laser beams deplete the emission, achieving the super-resolution effect by time-gating it. Given the WLL excitation and the three depletion lines, multicolour STED imaging is readily possible. Further details
Leica DMi8 with Codex
This inverted microscope is a flexible, multipurpose widefield imaging platform equipped with a Hamamatsu Orca Flash 4.0 V3 camera, a Lumencor SOLA-SE-II Light source (365nm Version) and an Okolab incubation chamber, fitted with temperature and CO2 control.
It is suitable for many applications from live cell imaging to automated slide tiling and Akoya Codex multiplex imaging. The Leica DMI8 is currently the only imaging system in the facility that allows imaging at the Cy7 light range with an excitation band pass filter at 710 nm +/- 75nm. This allows for starightforward and fast 5 colour imaging. The available excitation filter cubes are: DAPI [BP 355-425], GFP [BP 470/40], Y3 [BP 535/50], Y5 [BP 620/60] and Y7 [BP 710/75].
The DMI8 is optimized for automated imaging and offers several air objectives that don't require immersion media, which is ideal for imaging large multi-well plates or large specimen requiring a big field of view, which all require extensive stage movements that may otherwise lead to immersion media loss over time. The available objectives are: HC PL APO 10x/0.45, HC PL APO 20x/0.80, HC PL APO 40x/0.95 CORR.
The DMI8 microscope uses the Leica software platform LAS X (Leica Application Suite
X), which is capeable for a wide spectrum of fluorescence applications from routine work to sophisticated tasks such as z-stacking, stitching, deconvolution, large tiling and multiposition live cell timelapse imaging as well as real time 3D visualization.
The Leica DMI8 with CODEX technology is an automated flowthrough staining setup that enables coverslip mounted tissue sections to be repeatedly stained and imaged on the microscope stage. CODEX uses an antibody conjugated to proprietary Akoya Barcodes, comprised of a unique oligonucleotide sequence, which have to be purchased though Akoya (https://www.akoyabio.com/codextm/technology). The CODEX Assay targets specific barcodes with a dye-labeled Reporter like GFP, Cy3, Cy5 and Dapi for highly specific automated detection. Akoya’s CODEX Barcode technology enables an almost unlimited level of flexibility in the generation of highly multiplexed panels while maintaining the tissue integrity ensuring high quality data.
Leica TCS SPE inverted Confocal microscope
This system, mounted on an inverted microscope stand, is a basic confocal with 4 colour capability. The TCS (true confocal scanner) with SP detector is a patented true confocal point scanning system. It has highly sensitive, filter-free spectral detection design with computer controlled adjustable bandwidth (freely tunable between 430 to 750nm with 5 nm spectral resolution). The flexible laser incoupling allows the use of up to 4 lasers (3 visible and 405 nm). As the confocal has just one detector, only sequential channel scans can be carried out, but with the available Lambda-Scan mode a sequential spectral image series can be recorded due to the filter-free design.
DeltaVision Elite Live Cell Imaging System
© Martin PhelpsThe DeltaVision Elite system is an advanced widefield microscope ideal for live cell imaging. The system, based on an Olympus microscope with excellent optics coupled to a CoolSNAP HQ2 CCD camera, is fitted with temperature and CO2 control to ensure cell viability while imaging. The system boasts a powerful LED-based light source and a versatile filter collection offering a wide range of multicolour imaging possibilities. This system is equipped with a very accurate XYZ encoded stage that enables setting up multipoint, time-lapse experiments in an easy manner. Moreover, the software running the system allows for online deconvolution of the acquired images. Further details
Olympus IX83 TIRF System
© Martin PhelpsThe Olympus IX83 TIRF microscope is a Total Internal Reflection (TIRF) purpose-built imaging system. TIRF is a laser-based technique that restricts illumination to the immediate vicinity of the coverglass surface. This results in exceptional optical sectioning, in turn enabling imaging of the basal part of the cell at very high signal-to-noise ratio. The system is fitted with temperature and CO2 control for live cell experiments, and a highly sensitive Photometrics Evolve Delta EMCCD camera, with matching high magnification objectives to ensure optimal sampling, and a Photometrics Prime sCMOS camera.
Additionally, high power lasers (405, 488, 561, and 640 nm), camera sensitivity and system flexibility enable for STORM/PALM stochastic single molecule-detection super-resolution imaging techniques, given the right sample buffer. This technique optimally allows for resolutions beyond the 20 nm mark. The system is complemented by a powerful software application (CellSens) incorporating a module for complex experimental design. Further details
Zeiss LSM780 inverted confocal microscope
© Martin PhelpsThis Zeiss 780 is a conventional laser scanning confocal mounted on an inverted microscope. This makes a robust system ideal for basic imaging applications that require optimal optical sectioning such as fixed and live cells grown on a coverglass and fixed tissue sections.
The two traditional PMT detectors are complemented with an array of 32 GaAsP detectors with improved sensitivity in the 470-650 nm range. The wide range of laser lines available at the system and the sensitive detector array enable for spectral imaging, meaning that virtually any fluorophore can be imaged and characterised on this system. This gives ultimate flexibility for sample preparation. Moreover, the system is fitted with temperature and CO2 control, so live cell imaging experiments are also possible. Further details
Zeiss 780 LSM Multiphoton/confocal upright system
© Martin PhelpsThis Zeiss 780 is a conventional laser scanning confocal mounted on an upright microscope. System architecture is designed for imaging thicker samples such as whole tissue sections. This system boasts a series of dipping lenses with large working distances that enable deeper tissue imaging in live and fixed as well as optically cleared samples. To fully exploit this capability, the system is fitted with a tunable MaiTai laser (range of 690-1040 nm) suitable for multi-photon excitation. Multiphoton microscopy is based on the co-incidence of 2 photons of the same wavelength at the same spot of the sample, exciting the fluorophores present in a very small volume.
The traditional PMT detectors are complemented with an array of 32 GaAsP detectors with improved sensitivity in the 470-650 nm range for standard confocal mode. For multiphoton imaging, this system also has four non-descanned detectors (NDDs; 2 PMTs and 2 GaAsPs) to enable optimal sensitivity for photons originating from the deepest parts of the sample. Further details
Zeiss LSM 880 inverted confocal microscope with Airyscan detector
The Zeiss 880 is a laser scanning confocal mounted on an inverted microscope. Since the system is mounted on an inverted body, thin samples or cells grown on a coverglass fit perfectly. Moreover, the system is fitted with temperature and CO2 control, so live cell imaging experiments are possible. This system is ideal for imaging applications that require optimal optical sectioning (Z-stacking).
Apart from the 2 traditional PMT detectors complemented with an array of 32 GaAsP detectors with improved sensitivity and spectral imaging capability. This system uniquely boasts an Airyscan detector, which enables improved spatial resolution confocal imaging (1.4x better than conventional imaging), equivalent to 0.2 AU pinhole setting but with a sensitivity equivalent to a 1.25AU pinhole setting. The right software tools are incorporated to render Airyscan-acquired images. Further details
Zeiss Cell Observer Spinning Disk Confocal
The Spinning disk is a laser-based parallelized confocal system based on the rotation of a disk with multiple pinholes that are projected onto the sample. Upon disk rotation, the illumination spots scan the sample, while a camera records the resulting confocal picture. The fact that multiple illumination spots reach the sample simultaneously translates in faster and gentler imaging compared to laser scanning microscopes. Our spinning disk boasts a Hamamatsu Flash4.0 v2 sCMOS camera with exceptional sensitivity for optimal sampling. Alternatively a Zeiss AxioCam CCD camera can also be used.
Since the system is mounted on an inverted body, cells grown on coverglass or chambers fit well. Moreover, the combination of extra speed and lower phototoxicity with temperature and CO2 control makes this system the perfect choice for fast live cell time-lapse imaging. Additionally, the extra speed also means faster Z-stacking, although confocality (image sharpness) is only guaranteed for a small range of lenses (63x and 100x oil immersion lenses). Further details