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Equipment

We have Fluidigm C1 and Biomark systems for microfluidic analysis of single cells, and a 10X Genomics Chromium that scales to thousands of single cells. We also have robotics and automation capability for sample processing.

10X Chromium Droplet RNAseq System

Enables RNAseq of 200-10000 single cells per sample. Cells are partitioned into microfluidic droplets, similar to the InDrop and Dropseq techniques. Whole exome and T/B Cell receptor kits are available. Further information.

 

Fluidigm Biomark QPCR system

Single Cell© Martin PhelpsThe Biomark can run microfluidic chips for massively parallel QPCR of 1-100 cell samples or cDNA from larger samples. The system utilises low volumes of standard gene expression or genotyping Taqman probes for analysis and so is very flexible and can be very cost and time effective.

Different chip formats are available: 24 genes in 192 samples, 48 genes in 48 samples, or 96 genes in 96 samples. A flexible chip modular and reusable chip is also available for upto 72 samples. Further information.

 

Robotics Liquid Handlers - Beckman FxP/4000 and TTP Mosquito

Single Cell Facility© Martin PhelpsThese robots can automate most genomic assays to provide consistency and scalability and are particularly useful for large scale projects. Automated protocols currently available: Ampure Magnetic bead clean ups (96/384 plates), QPCR (96/384 plates), sample pooling and normalisation (multiple 96/384 plates), Smartseq RNAseq, plate reformatting (96 to 384 etc), Illumina Nextera library preparation. The Mosquito allows miniaturisation of assays to save on reagent cost. Assays that have been successfully miniaturised so far: QPCR, Illumina library prep, PicoGreen, Kapa Illumina library quantification, whole genome amplifications.

 

Agilent Bioanalyzer, Tapestation 4200, AATI Fragment Analyzer

These systems can be used to analyse tiny amounts of protein, RNA or DNA conveniently. The Fragment Analyser can analyse up to 192 samples at a time and has a 2bp DNA resolution enabling convenient genotyping. Further information.

Commonly analyzed nucleic acid samples include:

  • Genomic DNA
  • Large Fragment DNA
  • Small Fragment DNA
  • Cell-free DNA (cfDNA)
  • NGS Libraries (Illumina and PacBio)
  • Plasmid (supercoild and linear)
  • SSR/Microsatellites
  • PCR Amplicons
  • Restriction Enzyme Digests
  • Small RNA NGS Libraries
  • mRNA
  • sgRNA
  • Small RNA/microRNA
  • Ribo-Depleted Total RNA
  • Total RNA
  • FFPE extracted nucleic acids

 

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