All of the Facility’s flow cytometers have high-end technical specifications to facilitate the wide range of research applications carried out in the MRC WIMM including, amongst many other applications, phenotyping to study changes in cell types following disease or vaccination and measurement of markers that are involved in haematopoiesis.
Attune NxT Analysers
© Martin Phelps
The Facility has three Attune NxT Analysers equipped with 4 lasers (405, 488, 561, 640nm) and autosamplers for rapid cell acquisition directly from 96 or 384 well plates. The Attune NxT has unique built in acoustic focussing technology that allows superior data resolution at high sample flow rates, this is ideal for rare cell analysis.
LSR Fortessa, LSR Fortessa X20
The Fortessa and one X20 analyser are fitted with 4 lasers (405, 488, 561, 640nm). Each instrument can be used with a high-throughput sampler to automate sampling directly from 96 well plates. These analysers are highly sensitive and capable of assaying up to 16 colours. We also have a 5 laser X20 (355, 405, 488, 561, 640nm) which can analyse up to 18 colours.
LSR II, Symphony A3 (Fortessa X50)
The LSRII and X50 are both fitted with 5 lasers (355, 405, 488, 532 or 561, 640nm) to offer the widest possible choice of fluorochrome selection. Both instruments can be used with a high-throughput sampler to automate sampling directly from 96 well plates. The LSR II can assay up to 17 colours and the X50 is configured to measure up to 28 colours so it is the perfect choice for developing high-content panels.
Aria II, Aria III, Aria Fusion cell sorters
© Martin Phelps
The FACS Aria II is fitted with 4 lasers (405, 488, 532, 640nm), The Aria III and two Fusion sorters also have 4 lasers (405, 488, 561 and 640nm). All the instruments have automatic cell deposition devices for sorting into plates. All the sorters (except the Aria II) are installed inside biosafety cabinets for enhanced operator protection. 15-16 colours can be assayed making the sorters suitable for complex reagent panels.
The Sony MA900 is a fully automated cell sorter and is extremely easy to operate. It has 4 lasers and can assay up to 12 colours simultaneously. It has excellent ability to sort single cells into 96 and 384 well plates. Users may self-operate this instrument after a short training session.