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Pancreatic adenocarcinoma cell lines rarely express the CFTR gene, despite the high levels of CFTR protein that are present in primary pancreatic duct cells. We have attempted to generate a non-CF pancreatic adenocarcinoma cell line that stably produces high levels of CFTR mRNA and protein by transfecting a vector containing the CFTR cDNA, driven by a strong mammalian promoter, into the poorly differentiated pancreatic adenocarcinoma cell line, Panc-1. The pANS6 pancreatic duct cell line expresses substantial levels of CFTR mRNA, but little CFTR protein. Despite this we were able to detect low conductance chloride channels in 40% of patches, stimulated with cAMP, that have similar biophysical properties to CFTR.

Original publication

DOI

10.1016/0925-4439(95)00047-8

Type

Journal article

Journal

Biochim Biophys Acta

Publication Date

09/06/1995

Volume

1271

Pages

315 - 320

Keywords

Adenocarcinoma, Cell Line, Chloride Channels, Cyclic AMP, Cystic Fibrosis Transmembrane Conductance Regulator, Genetic Vectors, Membrane Proteins, Pancreatic Neoplasms, Patch-Clamp Techniques, RNA, Messenger, Transfection, Tumor Cells, Cultured