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In order to examine the interactions of the cAMP activated CFTR chloride ion channel with other chloride ion channels present in epithelial cells we have generated a model based on antisense mRNA down regulation of CFTR expression. The HT29-derived epithelial cell line CJC4-1 has integrated a plasmid that is constitutively expressing CFTR exons 1-6 in the antisense orientation, from the MoMULV LTR. This cell line shows a reduction in cAMP-activated chloride efflux, as measured by iodide efflux assay, in comparison to the parental HT29 cell line and a sense transfected control.

Original publication

DOI

10.1016/0167-4781(95)00043-g

Type

Journal article

Journal

Biochim Biophys Acta

Publication Date

26/04/1995

Volume

1261

Pages

417 - 423

Keywords

Cell Line, Cell Survival, Chloride Channels, Cystic Fibrosis, Cystic Fibrosis Transmembrane Conductance Regulator, Down-Regulation, Epithelium, Gene Expression Regulation, Humans, Membrane Proteins, Plasmids, RNA, Antisense, RNA, Messenger, Transfection