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A cDNA clone p5x23 was isolated from a library prepared from membrane bound mRNA from a human lymphoblastoid cell line. This clone was shown to detect a restriction fragment length polymorphism with the enzyme MspI, with frequency for the minor variant of about 10% in a Caucasian population. The clone apparently only detected homozygotes, and did not hybridize to any of a panel of human-mouse somatic cell hybrids containing all human chromosomes in various combinations. Sequence analysis of the clone showed that it encoded a part of the 3' end of the human mitochondrial cytochrome b gene.


Journal article


Mol Biol Med

Publication Date





239 - 249


Amino Acid Sequence, Base Sequence, Chromosome Mapping, Cloning, Molecular, Cytochrome b Group, DNA, DNA Restriction Enzymes, DNA, Mitochondrial, Gene Expression Regulation, Humans, Polymorphism, Genetic