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A previously described genomic library constructed from microdissected DNA has been used to generate a large number of probes around the adenomatous polyposis coli (APC) gene at 5q22. A total of 202 clones were hybridised directly onto a somatic cell hybrid panel containing two APC-related interstitial deletions. Of 75 microclones that gave clear hybridisation signals, 22 independent clones mapped into the region common to both deletions. In addition, 4/22 of the markers are conserved in rodent DNA. These clones should provide a valuable resource for screening cDNA libraries and cloning the DNA around the APC gene in yeast artificial chromosomes.

Original publication

DOI

10.1007/BF00204940

Type

Journal article

Journal

Hum Genet

Publication Date

11/1991

Volume

88

Pages

112 - 114

Keywords

Adenomatous Polyposis Coli, Cells, Cultured, Chromosome Mapping, Chromosomes, Human, Pair 5, Cloning, Molecular, Genomic Library, Humans