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Cells rely on versatile diffusion dynamics in their plasma membrane. Quantification of this often heterogeneous diffusion is essential to the understanding of cell regulation and function. Yet such measurements remain a major challenge in cell biology, usually due to low sampling throughput, a necessity for dedicated equipment, sophisticated fluorescent label strategies, and limited sensitivity. Here, we introduce a robust, broadly applicable statistical analysis pipeline for large scanning fluorescence correlation spectroscopy data sets, which uncovers the nanoscale heterogeneity of the plasma membrane in living cells by differentiating free from hindered diffusion modes of fluorescent lipid and protein analogues.

Original publication

DOI

10.1021/acsnano.8b04080

Type

Journal article

Journal

ACS Nano

Publication Date

28/08/2018

Volume

12

Pages

8540 - 8546

Keywords

Brownian, actin cytoskeleton, diffusion modes, free and trapped diffusion, hindered diffusion dynamics, scanning FCS