Interferon-induced transmembrane 3 (IFITM3) is known to restrict the entry of a range of enveloped viruses. The single nucleotide polymorphism rs12252-C within IFITM3 has been shown to be associated with severe influenza A virus infection. It has been suggested that rs12252-C results in expression of a truncated IFITM3 protein lacking the first 21 amino acids. By performing high-throughput RNA sequencing on primary dendritic cells and peripheral blood mononuclear cells isolated from pandemic H1N1 influenza and human immunodeficiency virus-1 (HIV-1) infected patients we show that full-length IFITM3 mRNA is dominantly expressed (>99%) across all rs12252 genotypes. Full-length IFITM3 protein can be detected in all genotypes.
J Infect Dis
257 - 262
IFITM3, influenza A virus, rs12252, Dendritic Cells, Humans, Influenza A Virus, H1N1 Subtype, Influenza, Human, Leukocytes, Mononuclear, Membrane Proteins, Polymorphism, Single Nucleotide, RNA, Messenger, RNA-Binding Proteins, Sequence Analysis, RNA, United Kingdom