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Flow cytometry and two-colour immunofluorescence were used to detect cytoplasmic bovine viral diarrhoea virus (BVDV) antigen in leukocytes from viraemic cattle. Monoclonal antibody to the p80 protein of BVDV, a non-structural viral antigen, was used to identify the subpopulations of leukocytes in which viral protein synthesis had occurred. Viral antigen was detected in 23% of peripheral blood mononuclear cells. Monocytes were found to have the highest frequency of infection (35%). A higher proportion of CD2+ T cells (23%) were infected, compared with B cells (11%) or WC1+ gamma delta T cells (11%). No significant differences in percentages of different leukocyte subpopulations in blood were detected in persistently viraemic animals compared with controls.

Original publication

DOI

10.1099/0022-1317-75-5-1189

Type

Journal article

Journal

J Gen Virol

Publication Date

05/1994

Volume

75 ( Pt 5)

Pages

1189 - 1194

Keywords

Animals, Antibodies, Monoclonal, Antibodies, Viral, Antigens, Viral, Bovine Virus Diarrhea-Mucosal Disease, Cattle, Flow Cytometry, Leukocytes, Monocytes, Viral Nonstructural Proteins, Viremia