A new phosphate-starvation response in fission yeast requires the endocytic function of myosin I.

Petrini E., Baillet V., Cridge J., Hogan CJ., Guillaume C., Ke H., Brandetti E., Walker S., Koohy H., Spivakov M., Varga-Weisz P.

Endocytosis is essential for uptake of many substances into the cell, but how it links to nutritional signalling is poorly understood. Here, we show a new role for endocytosis in regulating the response to low phosphate in Schizosaccharomyces pombe. Loss of function of myosin I (Myo1), Sla2/End4 or Arp2, proteins involved in the early steps of endocytosis, led to increased proliferation in low-phosphate medium compared to controls. We show that once cells are deprived of phosphate they undergo a quiescence response that is dependent on the endocytic function of Myo1. Transcriptomic analysis revealed a wide perturbation of gene expression with induction of stress-regulated genes upon phosphate starvation in wild-type but not Δmyo1 cells. Thus, endocytosis plays a pivotal role in mediating the cellular response to nutrients, bridging the external environment and internal molecular functions of the cell.

DOI

10.1242/jcs.171314

Type

Journal article

Journal

J Cell Sci

Publication Date

15/10/2015

Volume

128

Pages

3707 - 3713

Keywords

Endocytosis, Myosin, Phosphate sensing, Actin-Related Protein 2, Endocytosis, Gene Deletion, Gene Expression Regulation, Fungal, Myosin Heavy Chains, Phosphates, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Signal Transduction, Transcriptome

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