Pre-clinical modelling of chromosomal translocations and inversions
Ruggero K., Rabbitts TH.
© Springer International Publishing Switzerland 2015. Animal models of tumour formation induced by chromosomal translocations are unique tools to study the consequence and tumourigenic potential of these chromosomal abnormalities in a living animal. There are several approaches to generate mouse translocation models including microinjection into one-cell embryos of the translocation or fusion transgene, bone marrow transplantation models for leukaemia/ lymphoma, and gene targeting by homologous recombination for specific expression in chimaeric mice using embryonic stem cells. Other model organisms such as zebrafish are also available as alternatives to mice. In addition, new gene editing technologies promise to facilitate rapid generation of parallel models of human chromosomal abnormalities.