Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

RNA-driven complementary DNA (cDNA) hybridization experiments have been carried out in order to detect complementary sequences in RNA prepared from a mouse T cell lymphoma line (EL4). In conditions where efficient hybridization of L-chain cDNA with homologous P3 myeloma mRNA was observed, poor hybridization was observed with EL4 mRNA unless a low criterion of hybrid formation was employed (i.e. hydroxyapatite fractionation). The hybrid formed between EL4 mRNA and L-chain cDNA was found to melt about 5 degrees C below the homologous hybrid indicating that the sequence detected in EL4 mRNA is similar but not identical with the P3 mRNA sequence. However, a similar sequence was detectable in a Clambda-producing myeloma cell line but in this line the concentration of the sequence was found to be an order of magnitude lower than in EL4 cells.

Original publication




Journal article


Eur J Immunol

Publication Date





43 - 48


Base Sequence, DNA, Hot Temperature, Immunoglobulin Light Chains, Immunoglobulin kappa-Chains, Lymphoma, Neoplasms, Experimental, Nucleic Acid Denaturation, Nucleic Acid Hybridization, Oligonucleotides, RNA, Messenger, RNA-Directed DNA Polymerase, T-Lymphocytes, Transcription, Genetic