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Quantum dots are available in a range of spectrally separated emission colors and with a range of water-stabilizing surface coatings that offers great flexibility for enabling bio-specificity. In this study, we have taken advantage of this flexibility to demonstrate that it is possible to perform a simultaneous investigation of the lateral dynamics in the plasma membrane of i) the transmembrane epidermal growth factor receptor, ii) the glucosylphospatidylinositol-anchored protein CD59, and iii) ganglioside GM1-cholera toxin subunit B clusters in a single cell. We show that a large number of the trajectories are longer than 50 steps, which we by simulations show to be sufficient for robust single trajectory analysis. This analysis shows that the populations of the diffusion coefficients are heterogeneously distributed for all three species, but differ between the different species. We further show that the heterogeneity is decreased upon treating the cells with methyl-β-cyclodextrin.

Original publication

DOI

10.1371/journal.pone.0097671

Type

Journal article

Journal

Plos one

Publication Date

2014

Volume

9

Keywords

Animals, CD59 Antigens, Cell Survival, Cell Tracking, Computer Simulation, Diffusion, G(M1) Ganglioside, Imaging, Three-Dimensional, Mercaptoethanol, Mice, Monte Carlo Method, Quantum Dots, Receptor, Epidermal Growth Factor, Reproducibility of Results, Staining and Labeling, Streptavidin