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A system devised for tissue typing the HLA-A locus by PCR from genomic DNA has been used to investigate abnormalities of HLA expression in a panel of 30 colorectal tumor cell lines, by comparing the HLA-A locus genotype with surface expression of HLA. Three cell lines showed complete lack of HLA expression associated with failure to express beta 2-microglobulin. In two other cell lines, loss of expression of HLA-A2 was observed, in spite of the presence of the gene in genomic DNA. Eleven cell lines gave a single HLA-A locus specificity on PCR typing. In one of these cell lines we have demonstrated the loss of an HLA-A locus gene in the tumor cell by comparison with DNA from a lymphoblastoid B-cell line derived from the same patient. These data indicate that at least three independent mechanisms were involved in the loss of HLA expression on the colorectal tumor cell lines.

Type

Journal article

Journal

Proc natl acad sci u s a

Publication Date

01/04/1993

Volume

90

Pages

2842 - 2845

Keywords

Base Sequence, Colonic Neoplasms, Colorectal Neoplasms, DNA, Neoplasm, Gene Expression, Genotype, HLA-A Antigens, Histocompatibility Testing, Humans, Molecular Sequence Data, Oligodeoxyribonucleotides, Polymerase Chain Reaction, Tumor Cells, Cultured, beta 2-Microglobulin