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Antisera have been prepared, in mice and rabbits, to membrane and sub-membrane fractions of human B lymphocyte derived lymphoid lines. Antisera to a protein subfraction were, after only minimal absorption, specific for human peripheral B lymphocytes, monocytes and B cell derived lymphoid lines. The antigen(s) recognised by these antisera were not the same as the previously described B-cell markers; immunoglobulin, Fc receptor, complement receptor and Ia antigens. The antigen(s) could not be removed from cells by lysostrip with anti- beta2 microglobulin.

Type

Journal article

Journal

Tissue antigens

Publication Date

02/1976

Volume

7

Pages

105 - 117

Keywords

Absorption, Animals, Antibody Formation, B-Lymphocytes, Cell Line, Cell Membrane, Cells, Cultured, Complement System Proteins, Cytotoxicity Tests, Immunologic, Fluorescent Antibody Technique, HLA Antigens, Humans, Immune Adherence Reaction, Immune Sera, Mice, Monocytes, Rabbits, T-Lymphocytes