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Human fibroblasts containing a translocation between the X chromosome and chromosome 15 were fused with the 6-thioguanine-resistant mouse cell line, IR. Resulting hybrids, selected in HAT medium, retained the X/15 chromosome. Hybrids which were counterselected in 6-thioguanine lost this chromosome. The X-linked markers glucose-6-phosphate dehydrogenase (G6PD), phosphoglycerate kinase (PGK), and hypoxanthine phosphoribosyl transferase (HPRT), and the non-X-linked markers pyruvate kinase (PKM2) mannose phosphate isomerase (MPI), N-acetyl hexosaminidase A (HEXA) and beta2-microglubulin (beta2-m) all segregated in concordance with the X/15 translocation chromosome. The latter markers have been assigned to chromosome 15. Selection against the X/15 chromosome was done using antihuman beta2-m serum. Electrophoretic and immunochemical analyses of the N-acetyl hexosaminidases A and B in these hybrids were performed.

Type

Journal article

Journal

Somatic cell genet

Publication Date

03/1976

Volume

2

Pages

125 - 140

Keywords

Acetylglucosaminidase, Beta-Globulins, Carbohydrate Epimerases, Chromosome Mapping, Chromosomes, Human, 13-15, Genes, Genetic Linkage, Hexosaminidases, Humans, Hybrid Cells, Karyotyping, Mannose, Pyruvate Kinase, Sex Chromosomes, Sugar Phosphates, Translocation, Genetic, beta 2-Microglobulin