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The p53-inducible TIGAR protein functions as a fructose-2,6-bisphosphatase, promoting the pentose phosphate pathway and helping to lower intracellular reactive oxygen species (ROS). ROS functions in the regulation of many cellular responses, including autophagy--a response to stress conditions such as nutrient starvation and metabolic stress. In this study, we show that TIGAR can modulate ROS in response to nutrient starvation or metabolic stress, and functions to inhibit autophagy. The ability of TIGAR to limit autophagy correlates strongly with the suppression of ROS, with no clear effects on the mTOR pathway, and is p53 independent. The induction of autophagy in response to loss of TIGAR can function to moderate apoptotic response by restraining ROS levels. These results reveal a complex interplay in the regulation of ROS, autophagy and apoptosis in response to TIGAR expression, and shows that proteins similar to TIGAR that regulate glycolysis can have a profound effect on the autophagic response through ROS regulation.

Original publication

DOI

10.1038/emboj.2009.242

Type

Journal article

Journal

EMBO J

Publication Date

07/10/2009

Volume

28

Pages

3015 - 3026

Keywords

Apoptosis, Apoptosis Regulatory Proteins, Autophagy, Cell Line, Tumor, Gene Expression Regulation, Humans, Intracellular Signaling Peptides and Proteins, Protein Kinases, Reactive Oxygen Species, TOR Serine-Threonine Kinases, Tumor Suppressor Protein p53