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BTG2/TIS21/PC3 protein is involved in the regulation of G1/S transition of the cell cycle by inhibiting pRb function, suggesting that BTG2/TIS21/PC3 regulation is critical for normal cell growth and proliferation. To understand the regulatory mechanisms for the expression of BTG2/TIS21/PC3 we cloned the human gene. Potential binding sites for several transcription factors were identified in the 5'-flanking region of the gene. Transient expression assays with BTG2/TIS21/PC3 promoter deletions and electrophoretic mobility shift analysis identified a major wild-type p53 response element located -74 to -122 relative to the start codon. This genomic fragment was sufficient to constitute a promoter element in the presence of p53. The BTG2/TIS21/PC3 gene is an antiproliferative gene which maps within a chromosomal segment (1q32) frequently altered in breast adenocarcinomas. However, no mutations of BTG2/TIS21/PC3 were detected in breast cancer cells, suggesting that the inactivation of this gene is not a frequent genetic event during breast carcinogenesis.

Original publication

DOI

10.1016/s0378-1119(01)00825-3

Type

Journal article

Journal

Gene

Publication Date

09/01/2002

Volume

282

Pages

207 - 214

Keywords

Base Sequence, Gene Expression Regulation, Genes, Genes, Tumor Suppressor, Genetic Vectors, Genotype, Humans, Immediate-Early Proteins, Luciferases, Molecular Sequence Data, Mutation, Promoter Regions, Genetic, Recombinant Fusion Proteins, Transcription, Genetic, Transfection, Tumor Cells, Cultured, Tumor Suppressor Protein p53, Tumor Suppressor Proteins