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In cystic fibrosis (CF), transcript analysis and quantification are important for diagnosis, prognosis and also as surrogate markers for some therapies including gene therapy. Classical RNA-based methods require significant expression levels in target samples for appropriate analysis, thus PCR-based methods are evolving towards reliable quantification. Various protocols for the quantitative analysis of CFTR transcripts (including those resulting from splicing variants) are described and discussed here.

Original publication

DOI

10.1016/j.jcf.2004.05.047

Type

Journal article

Journal

J cyst fibros

Publication Date

08/2004

Volume

3 Suppl 2

Pages

17 - 23

Keywords

Cystic Fibrosis, Cystic Fibrosis Transmembrane Conductance Regulator, Genetic Techniques, Humans, Reverse Transcriptase Polymerase Chain Reaction, Transcription, Genetic