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The scope of this article is to outline some of the basic methods for good quality RNA preparation from mammalian tissues and cells (including epithelial cells). Additionally, we give an outline of common techniques of measuring CFTR gene expression such as quantitative and semi-quantitative reverse transcription (RT) PCR and ribonuclease protection assay (RPA). These methods are designed to detect low abundance transcripts, which apply to CFTR mRNA in most cell types and tissues.

Original publication




Journal article


J Cyst Fibros

Publication Date



3 Suppl 2


11 - 15


Cystic Fibrosis Transmembrane Conductance Regulator, DNA, Complementary, Gene Expression Profiling, Genetic Techniques, Humans, RNA, Reverse Transcriptase Polymerase Chain Reaction