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Cystic fibrosis transmembrane conductance regulator gene (CFTR) shows a complex mechanism of tissue-specific and temporal regulation. CFTR mRNA detection and measurement are extremely difficult because of the low to very low levels of its endogenous expression. In this paper, we describe four different non-PCR methods optimized to analyze CFTR transcripts in epithelial cell lines, primary cell lines and native tissues that express significant amounts of CFTR transcript.

Original publication

DOI

10.1016/j.jcf.2004.05.005

Type

Journal article

Journal

J cyst fibros

Publication Date

08/2004

Volume

3 Suppl 2

Pages

25 - 28

Keywords

Blotting, Northern, Cell Line, Cystic Fibrosis Transmembrane Conductance Regulator, DNA, Complementary, Epithelial Cells, Genetic Techniques, Humans, In Situ Hybridization, Nucleic Acid Amplification Techniques, Transcription, Genetic