The cortical actin network regulates avidity-dependent binding of hyaluronan by the lymphatic vessel endothelial receptor LYVE-1.

Stanly TA., Fritzsche M., Banerji S., Shrestha D., Schneider F., Eggeling C., Jackson DG.

Lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1) mediates the docking and entry of dendritic cells to lymphatic vessels through selective adhesion to its ligand hyaluronan in the leukocyte surface glycocalyx. To bind hyaluronan efficiently, LYVE-1 must undergo surface clustering, a process that is induced efficiently by the large cross-linked assemblages of glycosaminoglycan present within leukocyte pericellular matrices but is induced poorly by the shorter polymer alone. These properties suggested that LYVE-1 may have limited mobility in the endothelial plasma membrane, but no biophysical investigation of these parameters has been carried out to date. Here, using super-resolution fluorescence microscopy and spectroscopy combined with biochemical analyses of the receptor in primary lymphatic endothelial cells, we provide the first evidence that LYVE-1 dynamics are indeed restricted by the submembranous actin network. We show that actin disruption not only increases LYVE-1 lateral diffusion but also enhances hyaluronan-binding activity. However, unlike the related leukocyte HA receptor CD44, which uses ERM and ankyrin motifs within its cytoplasmic tail to bind actin, LYVE-1 displays little if any direct interaction with actin, as determined by co-immunoprecipitation. Instead, as shown by super-resolution stimulated emission depletion microscopy in combination with fluorescence correlation spectroscopy, LYVE-1 diffusion is restricted by transient entrapment within submembranous actin corrals. These results point to an actin-mediated constraint on LYVE-1 clustering in lymphatic endothelium that tunes the receptor for selective engagement with hyaluronan assemblages in the glycocalyx that are large enough to cross-bridge the corral-bound LYVE-1 molecules and thereby facilitate leukocyte adhesion and transmigration.

DOI

10.1074/jbc.RA119.011992

Type

Journal article

Journal

J Biol Chem

Publication Date

10/04/2020

Volume

295

Pages

5036 - 5050

Keywords

STED-FCS, actin, confocal microscopy, dendritic cell, endothelial cell, fluorescence correlation spectroscopy (FCS), fluorescence recovery after photobleaching (FRAP), hyaluronan, immune system, leukocyte, lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1), membrane biophysics, membrane receptor dynamics, receptor, sFCS, stimulated emission depletion (STED) microscopy, Actin Cytoskeleton, Cells, Cultured, Endothelium, Lymphatic, Endothelium, Vascular, Humans, Hyaluronan Receptors, Hyaluronic Acid, Vesicular Transport Proteins

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