The Repertoire of Serous Ovarian Cancer Non-genetic Heterogeneity Revealed by Single-Cell Sequencing of Normal Fallopian Tube Epithelial Cells.

Hu Z., Artibani M., Alsaadi A., Wietek N., Morotti M., Shi T., Zhong Z., Santana Gonzalez L., El-Sahhar S., KaramiNejadRanjbar M., Mallett G., Feng Y., Masuda K., Zheng Y., Chong K., Damato S., Dhar S., Campo L., Garruto Campanile R., Soleymani Majd H., Rai V., Maldonado-Perez D., Jones S., Cerundolo V., Sauka-Spengler T., Yau C., Ahmed AA.

The inter-differentiation between cell states promotes cancer cell survival under stress and fosters non-genetic heterogeneity (NGH). NGH is, therefore, a surrogate of tumor resilience but its quantification is confounded by genetic heterogeneity. Here we show that NGH in serous ovarian cancer (SOC) can be accurately measured when informed by the molecular signatures of the normal fallopian tube epithelium (FTE) cells, the cells of origin of SOC. Surveying the transcriptomes of ∼6,000 FTE cells, predominantly from non-ovarian cancer patients, identified 6 FTE subtypes. We used subtype signatures to deconvolute SOC expression data and found substantial intra-tumor NGH. Importantly, NGH-based stratification of ∼1,700 tumors robustly correlated with survival. Our findings lay the foundation for accurate prognostic and therapeutic stratification of SOC.

DOI

10.1016/j.ccell.2020.01.003

Type

Journal article

Journal

Cancer Cell

Publication Date

10/02/2020

Volume

37

Pages

226 - 242.e7

Keywords

fallopian tube, non-genetic heterogeneity, ovarian cancer, single-cell RNA sequencing, Cystadenocarcinoma, Serous, Epithelial Cells, Epithelium, Fallopian Tube Neoplasms, Fallopian Tubes, Female, Gene Expression Regulation, Neoplastic, Genetic Heterogeneity, Humans, Ovarian Neoplasms

Permalink Original publication