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Mucosal-associated invariant T (MAIT) cells recognize intermediates of the vitamin B2 biosynthetic pathway present in a variety of bacteria, presented by the monomorphic MR1 molecules. Because of their central role in shaping adaptive immunity through interaction with dendritic cells (DCs) and B cells, their manipulation can be of translational relevance. We describe a method to routinely isolate and maintain MAIT cells from peripheral blood and to investigate their activity using DC as targets.

Original publication

DOI

10.1007/978-1-0716-0207-2_8

Type

Journal article

Journal

Methods Mol Biol

Publication Date

2020

Volume

2098

Pages

125 - 139

Keywords

CD40L, MAIT, MR1 dendritic cells