We present a recombineering-based method for editing the genome of a temperate phage. The method uses the lambda Red recombination system to edit the genome of a lysogenized host with a prophage compatible with bacteriophage lambda. Linear DNA is used as the recombination substrate and antibiotic resistance is used as the basis for selection of recombinants. The method enables the genetic manipulation of a prophage in 3-5 days.
Chapter
2022-01-01T00:00:00+00:00
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Bacteriophage, Electroporation, Lambda red, Lysogenic, Recombineering, Temperate, Bacteriophage lambda, Escherichia coli, Lysogeny, Prophages, Recombination, Genetic