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Please find below Useful technical instructions

Setting Up Koehler illumination/ adjusting the condenser

If you use the brightfield/DIC channel on any widefield or confocal or spinning disc microscope, you should set up Koehler Illumination to get the best contrast for your image. This is irrelevant for confocal/fluorescence microscopy. Please contact the facility team ( to get a practical demonstration on the instrument you wish to work on.

Tutorial and background information from Zeiss how to set up Koehler illumination

How to set up Koehler illumination (3 min video)