Services
We provide the following services and training:
- Single cell RNAseq plate based assays e.g.Smartseq2&3, CelSeq2, FB5P-Seq (5' RNAseq), Target -Seq (3' RNASeq).
- Single cell RNAseq on any of our microfluidic platforms: Fluidigm C1, Chromium, BD Rhapsody, Bio-Rad ddSeq.
- Single cell ATAC SEQ and combined single cell RNA/ATACseq
- Single cell SNP/CNV/genotyping using MissionBio Tapestri.
- Single cell Proteomics, BD Rhapsody, Bio-Rad ddSeq, Drop-seq, Tapestri
- Standard RNASeq (small cell numbers or RNA) - Smartseq2/3 for full-length, 3' Pool-Seq for 3' Prime. FB5P-seq for 5' Prime.
- Any commercial genomics kit.
- Spatial transciptomics - 10XGenomics Visium, probe based assays, Slide-Seq.
- Targeted Gene Expression (Fluidigm Biomark, BD Rhapsody, 10XGenomics Chromium)
- CITE-SEQ/REAP-SEQ: RNA and Protein from the same cell using antiboodies
- Antibody Oligo conjugation service.
- Fixed cell RNASeq
- Illumina Nextseq Sequencing
- Robotics and Automation: QPCR, library prep, assay miniaturisation.
- FACS sorting available through the WIMM FACS Facility
Workflows for scRNA/snRNA seq:
