Reagents for HLA typing and ARMS-PCR
10X NH4 buffer 3 ml
100mM dNTPs 240ul
50mM MgCl2 1.2 ml
Water 19.6 ml
Dispense in 1ml aliquots and freeze at -20C.
10X 13 ml
100mM dNTPs 923ul
50mM MgCl2 5.19 ml
Water 30.89 ml
Dispense in 1080ul aliquots and freeze at -20° C.
To set up a 192 well HLA typing (2x96 well plates)
Bioline Taq 10ul
Aliquot 207ul into 8 wells of falcon plate and use repeating pipettor to add 8ul to each well of 192wp.