Occasional WIMM Seminars

Thu 1 Dec 2016 from 11:00 to 11:45

MRC Weatherall Institute of Molecular Medicine, Seminar room, Headington OX3 9DS

New approaches to the investigation of the interactions between enhancers and promoters during haemopoiesis

Dr James Davies

Audience: Members of the University only

Organisers: Liz Rose

VIVA SEMINAR

Fri 2 Dec 2016 from 13:00 to 14:00

MRC Weatherall Institute of Molecular Medicine, Seminar room, Headington OX3 9DS

Long-distance interactions in gene expression control: function and regulation by the 3D genome's architecture

Dr Francois Spitz

Audience: Members of the University only

Organisers: Liz Rose

Thu 8 Dec 2016 from 14:00 to 15:00

MRC Weatherall Institute of Molecular Medicine, Seminar Room, Headington OX3 9DS

Phosphorylated CtIP functions as a co-factor of the MRE11-RAD50-NBS1 endonuclease in DNA end resection

Professor Petr Cejka

To repair a DNA double-strand break (DSB) by homologous recombination (HR), the 5'-terminated strand of the DSB must be resected. The human MRE11-RAD50-NBS1 (MRN) and CtIP proteins were implicated in the initiation of DNA end resection, but the underlying mechanism remained undefined. Here we show... Read more

To repair a DNA double-strand break (DSB) by homologous recombination (HR), the 5'-terminated strand of the DSB must be resected. The human MRE11-RAD50-NBS1 (MRN) and CtIP proteins were implicated in the initiation of DNA end resection, but the underlying mechanism remained undefined. Here we show that CtIP is a co-factor of the MRE11 endonuclease activity within the MRN complex. This function is absolutely dependent on CtIP phosphorylation that includes the key cyclin-dependent kinase target motif at Thr-847. Unlike in yeast where the Xrs2/NBS1 subunit is dispensable in vitro, NBS1 is absolutely required in the human system. The MRE11 endonuclease in conjunction with RAD50, NBS1 and phosphorylated CtIP preferentially cleaves 5'-terminated DNA strands near DSBs. Our results define the initial step of HR that is particularly relevant for the processing of DSBs bearing protein blocks or secondary DNA structures.

Audience: Members of the University only

Organisers: Penny Berry

Tue 20 Dec 2016 from 11:00 to 12:00

MRC Weatherall Institute of Molecular Medicine, Seminar room, Headington OX3 9DS

Lineage commitment and chromatin dynamics

Professor Frank Grosveld

Audience: Members of the University only

Organisers: Liz Rose

Tue 20 Dec 2016 from 13:00 to 13:45

MRC Weatherall Institute of Molecular Medicine, Seminar room, Headington OX3 9DS

The role of elements binding CTCF and Cohesin in directing tissue-specific enhancer activity

Lars Hanssen

Audience: Members of the University only

Organisers: Liz Rose

VIVA SEMINAR